Cleaning Procedure For Biological Safety Cabinet

This may take more or less time depending on the disinfectant and the microbiological agent used.

Cleaning procedure for biological safety cabinet.

To begin apply disinfect to a towel and decontaminate and remove items from the area of the spill. Aseptic technique and daily decontamination will eliminate the majority of contaminants. In addition periodic and thorough decontamination routines including disinfecting all removable parts and surfaces is recommended by the manufacturer and is a good laboratory practice to reduce wear on the equipment and provide greater safety to you your samples and the environment. Cleaning a biosafety cabinet or bsc is an important function in terms of both containment and sterility.

Remember to wear appropriate ppe and leave the biosafety cabinet on during spill cleanup. Put the waste from the spill into a biohazard bag inside the cabinet. μ microns in diameter with higher efficiency for both larger and. 10 easy steps to cleaning a contaminated surface in the biosafety cabinet.

Spray the surfaces of all items placed into the cabinet with 70 ethanol to minimize the introduction of contaminants into the bsc. Allow to air dry. Hepa filters are defined as air cleaning devices that have a proven minimum removal efficiency of 99 97 of particles in the air equal to 0 3. The biohazard bag should never be kept outside the cabinet.

5 0 procedure 5 1 general cleaning procedure 5 1 1 ensure that the power supply of the biosafety cabinet is switched off. Typically surface decontamination is adequate however there are instances such as extensive repair inside the cabinet envelope or hepa filter replacement that will require gas decontamination. The interior surfaces should also be wiped with an appropriate disinfectant that would kill any microorganisms that could be found in the cabinet. How to clean a biological safety cabinet lafw hood.

5 1 3 once in a week remove adhered dust by wet mopping using soap solution. Afterward wipe the surface with a clean dry cloth to remove the moisture. If the biological safety cabinet is turned off between aseptic processes for routine maintenance or any other reason it should be operated long enough to allow complete purging of room air from the critical area for at least 30 minutes then cleaned and disinfected before use. 5 1 2 dedust the biosafety cabinet daily externally with a clean dry cloth.

How to clean a biological safety cabinet lafw hood. When work is completed all equipment and supplies from the bsc should be decontaminated and removed from the cabinet. It should be noted that the. Once the cabinet is empty you should allow 20 30 minutes to thoroughly decontaminate all interior work surfaces making sure to clean the back and side of the cabinet and the interior of the glass.

Once the cabinet has been operational for 15 minutes disinfect all interior surfaces of the cabinet thoroughly by spraying with 70 ethanol. The biological safety officer will determine the type of decontamination necessary.